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both first and second enantiomers to the R-PVPP molecule.
A.Preparation of First and Second Enantiomers
1) Preparation of 2-[(2-Amino-1-phenyl-ethyl)amino]-1-phenyl-ethan-1-ol (substrate) and Preparation of Benzimidazole (product)
The first step is the preparation of the S-enantiomer of the (R-1-phenylethyl)amino derivative. The racemic starting material 1-[(2-amino-1-phenyl-ethyl)amino]-2-phenyl-ethanol is reacted with an enantiomerically pure secondary amine to give the S-enantiomer and the racemic product. The racemic product is subjected to enantiomer resolution and isolated by means of a chiral HPLC column. In this isolation procedure, the racemic product is converted into the first enantiomer and the second enantiomer by enantiomeric exchange of a sodium salt as a base. The sodium salt is isolated by means of a chiral HPLC column and crystallized. The second step is the preparation of the corresponding benzimidazole (product). For this reaction, the first enantiomer is isolated by means of an enantiomeric separation and crystallized. To this end, the compounds 2-[(2-amino-1-phenyl-ethyl)amino]-1-phenyl-ethan-1-ol and 5-bromo-1-phenyl-2-benzimidazole are subjected to coupling reactions.
B) Preparation of the Third Enantiomer
As the first step, the third enantiomer is isolated by means of an enantiomeric separation and crystallized from the racemic mixture using the enantiomeric separation, by means of an enantiomeric separation or by means of a chiral HPLC column.
As the second step, the third enantiomer is prepared from the first
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Category:Audio codecs# Frozen-Assets on Heroku
A community-generated guide to [Heroku’s free-tier frozen-assets]( feature.
## What is a Frozen Asset?
A frozen-asset is a file which needs to be uploaded to your web server before you deploy your application.
This is similar to [Precompiled Assets](/guide/prep-assets) in the sense that you are compressing it during the build and freezing it during the deployment phase.
This allows you to freeze the assets in their production environment, but *not* compile them when generating the source code.
## What’s a Frozen Asset?
A frozen-asset is a file on your web server which your app is referencing.
If you are using the Rackup DSL to manage your application, this file is a `.rb` or `.rb.erb` file.
If you are using [Ruby on Rails]( this file is in your `assets` directory.
The frozen-asset is a compressed archive of the files you want to use. This archive gets uploaded to the web server, and when the app is started, it gets uncompressed and shipped to the browser.
It contains the file’s content as well as information about its encoding and compression.
## Why do I need a Frozen Asset?
A frozen-asset is great if your files are heavy and need to be compressed, because you don’t have to compress them yourself. If, for example, you have a `.css` file with a lot of font resources, you can freeze the whole `fonts` directory